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sgrna targeting gfp sequence  (Addgene inc)


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    Structured Review

    Addgene inc sgrna targeting gfp sequence
    Sgrna Targeting Gfp Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrna+targeting+gfp+sequence/pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W+(Plasmid+%2367980)/pmc12475092-257-12-16
    Average 94 stars, based on 38 article reviews
    sgrna targeting gfp sequence - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Transduction:

    Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers.
    Article Snippet: .. Cas9 editing efficiency assay Cells were transduced with lentiviruses expressing BFP and GFP, as well as sgRNA targeting GFP sequence (Addgene #67980)58. ..

    Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers
    Article Snippet: .. Cells were transduced with lentiviruses expressing BFP and GFP, as well as sgRNA targeting GFP sequence (Addgene #67980) . ..

    Expressing:

    Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers.
    Article Snippet: .. Cas9 editing efficiency assay Cells were transduced with lentiviruses expressing BFP and GFP, as well as sgRNA targeting GFP sequence (Addgene #67980)58. ..

    Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers
    Article Snippet: .. Cells were transduced with lentiviruses expressing BFP and GFP, as well as sgRNA targeting GFP sequence (Addgene #67980) . ..

    Sequencing:

    Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers.
    Article Snippet: .. Cas9 editing efficiency assay Cells were transduced with lentiviruses expressing BFP and GFP, as well as sgRNA targeting GFP sequence (Addgene #67980)58. ..

    Article Title: Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci
    Article Snippet: .. In order to construct an NGA Cas9 activity reporter, pLentiGuide-Puro (Addgene plasmid ID 52963) was modified to express GFP and an NGA-restricted sgRNA targeting GFP sequence (Addgene plasmid ID 87156; ). .. HEK293T cells were cultured with Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Omega Scientific) and 2% penicillin-streptomycin (Life Technologies).

    Article Title: EXO1 as a therapeutic target for Fanconi Anaemia, ZRSR2 and BRCA1-A complex deficient cancers
    Article Snippet: .. Cells were transduced with lentiviruses expressing BFP and GFP, as well as sgRNA targeting GFP sequence (Addgene #67980) . ..

    Construct:

    Article Title: Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci
    Article Snippet: .. In order to construct an NGA Cas9 activity reporter, pLentiGuide-Puro (Addgene plasmid ID 52963) was modified to express GFP and an NGA-restricted sgRNA targeting GFP sequence (Addgene plasmid ID 87156; ). .. HEK293T cells were cultured with Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Omega Scientific) and 2% penicillin-streptomycin (Life Technologies).

    Activity Assay:

    Article Title: Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci
    Article Snippet: .. In order to construct an NGA Cas9 activity reporter, pLentiGuide-Puro (Addgene plasmid ID 52963) was modified to express GFP and an NGA-restricted sgRNA targeting GFP sequence (Addgene plasmid ID 87156; ). .. HEK293T cells were cultured with Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Omega Scientific) and 2% penicillin-streptomycin (Life Technologies).

    Plasmid Preparation:

    Article Title: Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci
    Article Snippet: .. In order to construct an NGA Cas9 activity reporter, pLentiGuide-Puro (Addgene plasmid ID 52963) was modified to express GFP and an NGA-restricted sgRNA targeting GFP sequence (Addgene plasmid ID 87156; ). .. HEK293T cells were cultured with Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Omega Scientific) and 2% penicillin-streptomycin (Life Technologies).

    Modification:

    Article Title: Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci
    Article Snippet: .. In order to construct an NGA Cas9 activity reporter, pLentiGuide-Puro (Addgene plasmid ID 52963) was modified to express GFP and an NGA-restricted sgRNA targeting GFP sequence (Addgene plasmid ID 87156; ). .. HEK293T cells were cultured with Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Omega Scientific) and 2% penicillin-streptomycin (Life Technologies).



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